LOCUS       B3GNT6-pGEn1-DES        5632 bp    DNA     circular     22-JAN-2013
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    2977..3054
                     /note="TCM Sig Seq"
     misc_recomb     complement(3114..3142)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    3055..3078
                     /note="8xHis Tag"
     misc_feature    3085..3108
                     /note="StrepTag"
     misc_feature    4260..4801
                     /note="WPRE"
     misc_recomb     4222..4244
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(4808..5022)
                     /note="BGH pA"
     misc_feature    complement(5110..5239)
                     /note="3'-ITR"
     misc_feature    3143..3165
                     /note="TEV site"
     source          3166..4215
                     /dnas_title="B3GNT6-pGEn1-DEST"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:119334 IMAGE:40005856"
                     /tissue_type="PCR rescued clones"
                     /clone_lib="NIH_MGC_282"
                     /note="Vector: pCR-Blunt II-TOPO; Clone identification
                     sequence tag: GCCTTATA sequenced from the reverse primer"
     gene            3166..4215
                     /dnas_title="B3GNT6"
                     /gene="B3GNT6"
                     /gene_synonym="B3Gn-T6"
                     /gene_synonym="IMAGE:4907098"
                     /db_xref="GeneID:192134"
                     /db_xref="HGNC:24141"
     CDS             3166..4215
                     /dnas_title="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 6 (core 3
                     synthase)"
                     /gene="B3GNT6"
                     /gene_synonym="B3Gn-T6"
                     /gene_synonym="IMAGE:4907098"
                     /codon_start=1
                     /product="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 6 (core 3
                     synthase)"
                     /protein_id="AAI03909.1"
                     /db_xref="GI:74353652"
                     /db_xref="GeneID:192134"
                     /db_xref="HGNC:24141"
                     /translation="MAFPCRRSLTAKTLACLLVGVSFLALQQWFLQAPRSPREERSPQ
                     EETPEGPTDAPAADEPPSELVPGPPCVANASANATADFEQLPARIQDFLRYRHCRHFP
                     LLWDAPAKCAGGRGVFLLLAVKSAPEHYERRELIRRTWGQERSYGGRPVRRLFLLGTP
                     GPEDEARAERLAELVALEAREHGDVLQWAFADTFLNLTLKHLHLLDWLAARCPHARFL
                     LSGDDDVFVHTANVVRFLQAQPPGRHLFSGQLMEGSVPIRDSWSKYFVPPQLFPGSAY
                     PVYCSGGGFLLSGPTARALRAAARHTPLFPIDDAYMGMCLERAGLAPSGHEGIRPFGV
                     QLPGAQQSSFDPCMYRELLLVHRFAPYEMLLMWKALHSPALSCDRGHRVS"
     misc_feature    3166..4215
                     /note="B3GNT6 coding region"
     misc_difference 4010..4011
                     /dnas_title="B3GNT6"
                     /gene="B3GNT6"
                     /gene_synonym="B3Gn-T6"
                     /gene_synonym="IMAGE:4907098"
                     /note="1 base in the human genome, T, is not found in
                     cDNA."
     misc_difference 4051..4052
                     /dnas_title="B3GNT6"
                     /gene="B3GNT6"
                     /gene_synonym="B3Gn-T6"
                     /gene_synonym="IMAGE:4907098"
                     /note="1 base in the human genome, T, is not found in
                     cDNA."
     misc_difference 4070..4071
                     /dnas_title="B3GNT6"
                     /gene="B3GNT6"
                     /gene_synonym="B3Gn-T6"
                     /gene_synonym="IMAGE:4907098"
                     /note="1 base in the human genome, T, is not found in
                     cDNA."
     vector          join(4230..5632,1..3132)
                     /source="pGEn1-DEST"
                     /type="Destination Vector"
     clone           3133..4229
                     /source="B3GNT6-Nterm-TEVfusion-pDONR221"
                     /type="Entry Clone"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC
     3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC
     3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA
     3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcaggtc cccgcgggag
     3181 gagaggtccc cgcaggagga gacgccagag ggtcccaccg acgctcccgc ggctgacgag
     3241 ccgccctcgg agctcgtccc cgggcccccg tgcgtggcga acgcctcggc gaacgccacg
     3301 gccgacttcg agcagctgcc cgcgcgcatc caggacttcc tgcggtaccg ccactgccgc
     3361 cacttcccgc tgctttggga cgcaccggcc aagtgcgccg gcggccgagg cgtgttcctg
     3421 ctcctggcgg tgaagtcggc gcctgagcac tacgagcgac gcgagctcat ccggcgcacg
     3481 tgggggcaag agcgcagcta cggcgggcgg ccagtgcgcc gcctctttct attgggcacc
     3541 ccgggccccg aggacgaggc gcgcgcggag cggctggcgg agctggtggc gctggaggcg
     3601 cgcgagcacg gcgacgtgct gcagtgggcc ttcgcggaca ccttcctcaa cctcacgctc
     3661 aagcacctgc acttgctcga ctggctggct gcacgctgcc cgcacgcgcg ctttctgctc
     3721 agcggcgacg acgacgtgtt cgtgcacacc gccaacgtag tccgcttcct gcaggcgcag
     3781 ccacccggcc gccacctgtt ctccggccag ctcatggagg gctccgtgcc catccgcgac
     3841 agctggagca agtacttcgt gccgccgcag ctcttccccg ggtccgctta cccggtgtac
     3901 tgcagcggcg gcggcttcct cctgtccggc cccacggccc gggccctgcg cgcggccgcc
     3961 cgccacaccc cgctcttccc catcgacgac gcctacatgg gcatgtgtct ggagcgcgcc
     4021 ggcctggcgc ccagcggcca cgagggcatc cggcccttcg gcgtgcagct gcctggcgca
     4081 cagcagtcct ccttcgaccc ctgcatgtac cgcgagttgc tgctagtgca ccgcttcgcg
     4141 ccctacgaga tgctgctcat gtggaaggcg ctgcacagcc ccgcgctcag ctgtgaccgg
     4201 ggacaccggg tctccTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAG CTTGGATCCA
     4261 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC
     4321 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA
     4381 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT
     4441 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG
     4501 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA
     4561 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT
     4621 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG
     4681 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA
     4741 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC
     4801 GAGATCTGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT
     4861 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT
     4921 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG
     4981 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT
     5041 TGCCGATTAG GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA
     5101 TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC
     5161 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG
     5221 TGAGCGAGCG AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC
     5281 TTCCGCCAAT TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC
     5341 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC
     5401 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG
     5461 CGCAGCCTGA ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA
     5521 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT
     5581 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG
//

